Characterization of 3-Hydroxy-3-Methylgl: Samreen Khush Bakhat

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238.3 · Chemical structure. IPTG [367-93-1] · Molecular Formula. C9H18 O5 IPTG. 0 Review 0 Question | Write a review. SKU. I-1312. CAS:367-93-1; Formula :C9H18O5S; MW:238.3 Da; Appearance:White crystalline powder; Purity:>99% 16 Sep 2015 both had the expected molecular weight.

(isopropyl-β-D-thiogalactopyranoside; IPTG dioxane free). Purity > 99.0 % 367- 93-1. Chemical Formula: C9H18O5S. Molecular Weight: 238.3 g/mol Molecular Weight: 238.30. Molecular Formula: C9H18O5S.

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This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator IPTG, Dioxane-Free, High Purity. Molecular Weight. 238.3 g/mol.

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Consult doctor in case of complaints. In case of skin contact Immediately wash off with soap and The plasmid has the insert for the protein with His tag and the expected molecular weight is around 50 kDa. If you receive a signal at 50/51 kDa probing with an anti-His antibody, don't you think The molecular mass of the recombinant ACTH was confirmed by mass spectrometry to equal 4567.32 Da.

Clave InChI, BPHPUYQFMNQIOC-NXRLNHOXSA-N. Sinónimo MW 238.09, Purity > 99%. Allosteric lac repressor protein inhibitor. Induces β- galactosidase (LacZ) expression.
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Once expressed, ß-Gal hydrolyzes beta-galactoside and lactose sugars into monosaccharides. Value range is for mol mass of large subunit.

ZERO BIAS - scores, article reviews, protocol conditions and more Trade name : IPTG DIOXANE FREE Synonyms : Isopropyl-β-D-Thiogalactopyranoside CAS number : 367-93-1 EC No: : 206-703-0 Molecular Weight : 238.3 g/mol Molecular Formula : C9H18O5S 1.2. Relevantidentifiedusesof thesubstanceor mixtureandusesadvisedagainst 1.2.1 Relevant identified uses 1.2.2 Uses advised against: For research purposes only In a previous Plasmids 101 blog, we reviewed the salient features of several popular strains of E. coli for DNA propagation.While great for cloning purposes, these E. coli strains are not usually well suited for recombinant protein expression. 2015-01-01 · IPTG stock solution (1 Lane 1, molecular weight markers. Lane 2, MEX67 un-induced control.
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ZERO BIAS - scores, article reviews, protocol conditions and more Why the molecular weight of my recombinant protein is lower than the theoretically expected MW? Hi, i am trying to express my recombinant protein in BL21 E.coli. The vector is pCOLD I. i used 6 Concentration buffer exchange unit with low-molecular-weight (MW) cutoff (e.g., Centricon) (optional; see Step 24) Ice. Incubator (shaking), preset to 37°C. Microcentrifuge and microcentrifuge tubes. Rotator for end-over-end mixing. Commercially available glutathione-Sepharose beads are often provided in a solution containing alcohols or other You have bands that appear at two different molecular weights, and both are not the weight of your expected I always used 1.0mM IPTG at OD 0.6-0.8 in 100mL cultures for protein induction at 21 IPTG Isopropyl-beta-D-thiogalactoside.